Competitive inhibitor test
Without inhibitor, Vmax=40 units/min and Km=2 mM. With inhibitor, Vmax=40 and apparent Km=6 mM.
- Vmax is unchanged.
- More substrate is needed for half-maximal rate.
- That pattern fits competition for the active site.
macromolecules, enzymes, and reaction conditions
Without inhibitor, Vmax=40 units/min and Km=2 mM. With inhibitor, Vmax=40 and apparent Km=6 mM.
Pause. Find what matters. Then make your move.